Erythropoietin sensitivity of rat bone marrow cells separated by velocity sedimentation.

Cover of: Erythropoietin sensitivity of rat bone marrow cells separated by velocity sedimentation. | Robert John Hamilton Miller

Published in [Toronto] .

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  • Erythropoietin,
  • Cell differentiation

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Book details

ContributionsToronto, Ont. University. Theses (M.Sc.)
LC ClassificationsLE3 T525 MSC 1969 M56
The Physical Object
Pagination[34,13 leaves]
Number of Pages3413
ID Numbers
Open LibraryOL18170950M

Download Erythropoietin sensitivity of rat bone marrow cells separated by velocity sedimentation.

Rat bone marrow cells have been separated on the basis of their sedimentation at unit gravity. The cell population most responsive to erythropoietin in vitro was found to have a sedimentation velocity of about mm/hr. In the process of becoming hemoglobin‐synthesizing cells, it undergoes cell division and its sedimentation velocity decreases to mm/hr and then to mm/hr, the sedimentation Cited by: 1.

Cell Tissue Kinet. Jan;3(1) Erythropoietin sensitivity of rat bone marrow cells separated by velocity sedimentation. McCool D, Miller RJ, Painter RH, Bruce by: ERYTHROPOIETIN SENSITIVITY OF RAT BONE MARROW CELLS SEPARATED BY VELOCITY SEDIMENTATION. Dorothy McCool; R.

Miller; R. Painter; W. Bruce; Pages: ; First Published: January ; Abstract; Full text PDF PDF References; Request permissions; AREA RELATIONS BETWEEN LABELING INDEX CURVES FROM MULTICOMPARTMENT CELL. A similar heterogeneity may exist among goat colonies. Thus, when goat bone marrow was fractionated by unit gravity sedimentation, more hemoglobin C synthesis was observed in colonies derived from cells of intermediate sedimentation velocity than in colonies derived from the most rapidly sedimenting cells.

Erythropoietin sensitivity of rat bone marrow cells separated by velocity sedimentation. Cell Tissue Kinet. Cell-cycle properties and proliferation kinetics of late erythroid progenitors in murine bone marrow. Suppression of mitogen-indueed peripheral B cell differentiation by soluble Fc y receptors released from lymphocytes.

Isokinetic Gradient Sedimentation of Human Bone Marrow Cells V. Concluding Remarks References I. Introduction The cellular composition of bone marrow, in comparison to other or gans, exhibits a remarkable heterogeneity due to the variety of progenitor cells involved in hematopoiesis, each of which has a unique developmen tal by: 1.

Sheep colonies behaved differently from those of goat in that little or no betaC globin synthesis occurred even at high erythropoietin concentration. To investigate this difference, sheep marrow cells were fractionated by unit gravity sedimentation.

Erythropoietin stimulates the rate of RNA synthesis by fetal mouse liver erythroid cell cultures. This effect is demonstrable prior to hormone-mediated effects on DNA synthesis. Various cell populations in rat bone marrow were characterized by means of a two dimensional separation using velocity sedimentation and free flow electrophoresis and by electrical sizing of the separated cells.

Up to mm/hr five different populations with discrete distributions in volume (coefficient of variation 10% to 13%) and sedimentation velocity (coefficient of variation 6% to 10% Cited by: Velocity sedimentation.

Cells were separated by means of velocity sedimentation according to Miller and Phillips (11) using a slightly modified technique as described (12). The sedimentation profiles were corrected for viable nucleated cells evaluated using the trypan blue exclusion test.

Erythropoietin is essential for bone marrow erythropoiesis and erythropoietin receptor on non-erythroid cells including bone marrow stromal cells suggests systemic effects of erythropoietin Cited by: 2. McCool D et al. () Erythropoietin sensitivity of rat bone marrow cells separated by velocity sedimentation.

Cell Tissue Kinet –65 PubMed Google Scholar McDonald JD et al. () Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin by: 4. Comparison of the Sysmex XTiV and microscopic bone marrow differential counts in Wistar rats treated with cyclophosphamide, erythropoietin, or serial phlebotomy.

It was found that the target cells which could be transformed in vitro by AEV were cells of light density (– g/cm 3), having a modal sedimentation velocity at unit gravity between and mm/hr, expressing an immature antigen at a low level and a brain-related antigen at a high level.

Bosi A,Vannucchi AM, Grossi A. Guidi S,Vannucchi L, Saccardi R, Bernabei PA, Longo G. Rafanelli D, Rossi Ferrini P (a) Serum erythropoietin levels in patients undergoing autologous bone marrow transplantation.

Bone Marrow Transplant 7: – PubMed Google ScholarCited by: 1. radiated bone marrow decreased as the dose to the marrow was increased, as shown in Fig.

The straight line shown is a least-squares fit to the data. To construct a survival curve for bone marrow cells from Fig. and 2. B, it is only necessary to assume that, for a given level of survival conferred on the irradiated recipient mice.

J Lab Clin Med ; 4. Pieber-Perretta M, Rudolph W, Perretta M, Hodgson G. Effect of erythropoietin on 14C-formate incorporation into rat bone ma- rrow-RNA.

Biochem Biophys Acta ; 5. Rudolph W, Perretta M. Effects of erythropoietin on 14C-formate uptake by spleen and bone marrow nucleic acids of erythrocyte- transfered Author: Marco Perretta, Fernando Garrido, Patricia Escárate.

The erythropoietic effect of various thyroid hormones has been studied using erythroid colony formation by canine marrow cells. Although erythropoietin was required for colony growth, physiologic levels of thyroid hormones significantly enhanced colony numbers. The order of potency of the thyroid compounds in their in vitro erythropoietic effect parallels their known calorigenic potency in vivo, suggesting that the in vitro effect Cited by: Effect of erythropoietin (EPO) on the localization of bone marrow mesenchymal stem cells (BMSCs) transplanted into spinal cord injury (SCI) sites 28 days following SCI in (A) the control group, (B) the BMSC treatment group and (C) the BMSC + EPO group.

GFP-labeled BMSCs were observed at the site of SCI in the BMSC and BMSC + EPO by: 1. Wu, J. et al. TGF-β1 induces senescence of bone marrow mesenchymal stem cells via increase of mitochondrial ROS production. BMC Developmental Biology.

14, /X Cited by: At the level of individual cells, bone marrow reticulocytes have been examined in situ by electron microscopy 7,8 and autoradiography 9 or in vitro by physiological function. 4,10,11 One study estimated that reticulocyte residency in the bone marrow prior to entering the circulation varies from 17 hours in normal rats to hours in anemic Cited by: dose- and time-dependent biphasic effects on bone nodule formation.

stimulating at low doses and at early stages of osteoblast differentiation but inhibiting at high doses and later; suggesting that PDGF has pleiotropic effects on bone metabolism, and that its effects on bone might be direct on osteoblast lineage cells. Together, my data validate methods for enrichment of osteoprogenitors Author: Alexandra Lenore Herbertson.

The broad use of transgenic and gene-targeted mice has established bone marrow-derived macrophages (BMDM) as important mammalian host cells for investigation of the macrophages biology.

Over the last decade, extensive research has been done to determine how to freeze and store viable hematopoietic human cells; however, there is no information regarding generation of BMDM from Cited by: examine the bone marrow, hemoglobin levels & reticulocyte count total erythropoiesis is assessed from the myeloid: erythroid ratio which is the proportion of granulocyte precursors to red cell precursors in bone marrow.

the normal range for this ratio is 1 to 1. Ferrokinetic data corresponded well with the response to erythropoietin in vitro. In the nonresponsive group, a recovery of sensitivity to erythropoietin was observed when the patients improved clinically. The nature of the bone marrow failure was discussed in relation to erythropoietin and stem by: In cell culture, used to separate live from dead cells.

Histopaque ® Suitable for separation of mononuclear cells from rat, mouse, or other mammalian peripheral blood or bone marrow. Histopaque ® Used in conjunction with Histopaque for the separation of granulocyes and mononuclear cells from human peripheral blood or bone marrow.

Erythropoietin sensitivity of rat bone marrow cells separated by velocity sedimentation McCool, McCool; Miller, Miller; Painter, Painter; Bruce, Bruce Erythropoiesis in the dog.

THE PROCESS OF RED BLOOD CELLS (ERYTHROPOIESIS) IN BONE MARROW. (IN DETAIL) THE PROCESS OF RED BLOOD CELLS (ERYTHROPOIESIS) IN BONE MARROW. Erythropoietin (EPO) - Duration: Medicosis. We studied the specific binding of I‐labeled bioactive recombinant human erythropoietin (Epo) to human bone marrow mononuclear cells (BMNC) obtained from normal subjects.

The I‐labeled Epo bound specifically to the BMNC. Scatchard analysis of the data showed two classes of binding sites; one high affinity (Kd nM) and the other low affinity (Kd nM).Cited by: Start studying Ch11 Blood.

Learn vocabulary, terms, and more with flashcards, games, and other study tools. - Erythropoietin-induced adaptive mechanism to improve blood's oxygen-carrying capacity in response to prolonged reduced oxygen delivery to the tissues - Originate from the same undifferentiated multipotent stem cells in red bone.

Summary. In answer to the need for a less time‐consuming assay of erythropoietin, the establishment of an in‐vitro method using foetal mouse liver cells is described. This could be completed in 26 hr and was capable of detecting erythropoietin in concentrations as low as or less International Units/ml.

It was found to be useful both for clinical measurements and as a system for the. Erythropoietin induces bone marrow and plasma fibroblast growth factor 23 during acute kidney injury Luis Toro1,2,3,Vı´ctor Barrientos1,4, Pablo Leo´n1, Macarena Rojas1, Magdalena Gonzalez1, Alvaro Gonza´lez-Iba´n˜ez4,5, Sebastia´n Illanes6, Keigo Sugikawa7,Ne´stor Abarzu´a1,Ce´sar Bascun˜a´n1, Katherine Arcos1, Carlos Fuentealba1, Ana Marı´a Tong8, Alvaro A.

Elorza4,5, Marı´a. Original Article Research of osteoblastic induced rat bone marrow mesenchymal stem cells cultured on β-TCP/PLLA porous scaffold Yi Yang1*, Jiang Wu2*, Gele Jin 1, Liang Li2, Zhongwei Li, Cao Li1 1Orthopedic Center, The First AffiliatedHospital of Xinjiang.

Within bone, mesenchymal stromal cells (MSCs) exist within the bone marrow stroma (BM-MSC) and the endosteal niche, as cells lining compact bone (CB-MSCs).

This study isolated and characterised heterogeneous MSC populations from each niche and subsequently investigated the effects of extensive cell expansion, analysing population doublings (PDs)/cellular senescence, colony-forming efficiencies Cited by: 5. Figure 2. Erythropoietin (EPO) upregulated fibroblast 23 (FGF23) via EPO receptor (EPOR) in vitro and in vivo.

(a–c) Mouse bone marrow (BM) cells were isolated from the tibiae of male C56BL/7 mice 8 to 12 weeks old (N = 5 per group).After 30 minutes, the medium was exchanged for medium containing EPO, EMP9 (EPOR homodimer antagonist), EPO+EMP9, ARA (EPOR/CD heterodimer agonist), or Cited by: Bone marrow cells (BMC) flushed from femora of Lewis rats were cultured in Dulbecco's modification of Eagle's medium supplemented with mouse L cell supernatant as a source of colony‐stimulating factor (CSF).

Differentiation of macrophage progenitor cells into macrophages (Mø) and expression of various markers were kinetically by: cation. Bone marrow cells were harvested by flushing the marrow cavity with Dulbecco's modified Eagle's medium (DMEM) (GIBCO).

The suspended cells were then collected by centrifugation at 1, rpm for 5 minutes. The cells were resus-pendedandculturedinL-DMEMmediawith10%fetalcalfserum (FCS) and 1% penicillin-gentamicin at 37 C in a humidified.

Analysis of the total cell population from the bone marrow by flow cytometry using MAb AA4 to immunolabel the mast cells showed that only ± % of the cells in the bone marrow are mast cells. Figure 4 shows the results of a representative experiment. By light microscopy, the number of mast cells present in the bone marrow was ± %.Cited by: The pluripotent hemopoietic stem cell (HSC) of the rat can be enumerated in a spleen colony assay (SCA) in rats as well as mice.

After injection of rat bone marrow into lethally irradiated mice, macroscopically visible spleen colonies (CFU-S) are found from day 6 thro but the number varies on consecutive days.

The mean cell volume (MCV) in the cord blood of full-term newborns is 90– fl. Erythropoieisis in fetal BM appears to be regulated by erythropoietin produced extrarenally, probably in the liver. From the 6th month there is also proliferation of HSC in the fetal liver which generate erythroid, myeloid and some lymphoid cells.

Bone Marrow Smear – The differential Practical advices • Always do your differential with 1,x magnification.

• • Count at least nucleated cells • Between cells can be counted in one field with oil immersion. • It becomes difficult with or more cells, as we tend to skip or to count the same cells twice. Comparison of CD44 and CD71 expression in dual-stained bone marrow cells.

Primary bone marrow cells were simultaneously stained with Ter, CD44, and CD (A) Plot of CD44 versus FSC of all TER positive cells. Note that 5 distinct clusters can be distinguished. (B) The CD44 expression levels in the gated cell by:   Bone marrow - nonneoplastic - Erythroid maturation (erythropoiesis) Erythropoietin (EPO): lineage specific cytokine (glycoprotein) responsible for erythropoiesis (proliferation, differentiation and survival by delaying apoptosis), produced by renal cells in response to hypoxia and by the bone marrow.

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